在体外，作为肾上腺髓质细胞的存活增强因子，睫状神经营养因子（CNTF）优于神经生长因子（NGF）。

CNTF is superior to NGF as a survival enhancement factor for adrenal medulla cells in vitro.

作者信息

Tokiwa M A, Gaspar E M, Doering L C

机构信息

Division of Anatomy, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada.

出版信息

Neuroreport. 1994 Jan 31;5(5):549-52. doi: 10.1097/00001756-199401000-00004.

DOI:10.1097/00001756-199401000-00004

PMID:8025241

Abstract

The effects of recombinant ciliary neurotrophic factor (CNTF) on chromaffin cells of the postnatal rat adrenal medulla were investigated and compared with recombinant nerve growth factor (NGF). Adrenal medulla cells were cultured in serum-free media and the survival was assessed by tyrosine hydroxylase immunohistochemistry after 4 days in vitro. While both recombinant NGF and CNTF enhanced the survival and differentiation of chromaffin cells, CNTF at 50-500 ng ml-1 was significantly better than NGF. Furthermore, CNTF was effective at 1.0 ng ml-1, and this effect was blocked with an antibody to CNTF. When the factors were combined, cell survival was also enhanced above the values for either growth factor alone. The highest proportion of cells induced to develop processes (neuronal phenotype) was obtained in the presence of CNTF.

摘要

研究了重组睫状神经营养因子（CNTF）对新生大鼠肾上腺髓质嗜铬细胞的作用，并与重组神经生长因子（NGF）进行了比较。将肾上腺髓质细胞培养于无血清培养基中，体外培养4天后通过酪氨酸羟化酶免疫组织化学评估细胞存活情况。虽然重组NGF和CNTF均能提高嗜铬细胞的存活率并促进其分化，但浓度为50 - 500 ng/ml的CNTF效果明显优于NGF。此外，1.0 ng/ml的CNTF也有效果，且这种效果可被抗CNTF抗体阻断。当两种因子联合使用时，细胞存活率也高于单独使用任一生长因子时的数值。在CNTF存在的情况下，诱导细胞形成突起（神经元表型）的比例最高。