Semchuk G M, Allen O B, O'Connor D L
Division of Applied Human Nutrition, University of Guelph, Ontario, Canada.
J Nutr. 1994 Jul;124(7):1118-25. doi: 10.1093/jn/124.7.1118.
This study was designed to assess the effect of succinylsulfathiazole on the apparent bioavailability of folate added to milk-containing diets. We also evaluated the impact of milk type on the relative bioavailability (bioavailability) of folate following pasteurization (62.5 degrees C, 30 min) and on the bioavailability of folic acid (PteGlu) vs. pteroylhexa-gamma-L-glutamic acid (PteGlu6). Following folate depletion (5 wk), 108 rats (six per group) were fed for 4 wk diets with or without 20 g milk solids/100 g diet and containing PteGlu, PteGlu + 5 g/kg succinylsulfathiazole, or PteGlu6. Folate bioavailability was determined using plasma folate concentration and a standard curve generated from rats fed milk-free diets with graded levels of PteGlu. The PteGlu and PteGlu6 bioavailability from human milk-containing diets was twice that of diets containing cow or goat milk (P < 0.05). Incorporation of a sulfa drug into diets containing human or cow milk reduced PteGlu bioavailability by one half (P < 0.05). Further, the values for bioavailability of PteGlu from diets containing human or goat milk no longer differed (0.86 and 0.75, respectively), and bioavailabilities from human milk- and goat milk-containing diets were greater than that of the cow-milk-containing diet (0.54) (P < 0.05). Pasteurization of milk did not influence folate bioavailability. The bioavailability of PteGlu6 was 49-71% that of PteGlu (P < 0.05). In summary, milk type differentially affects intestinal folate biosynthesis, and the superior folate bioavailability from human milk-containing diets is due in part to enhanced intestinal biosynthesis of folate.
本研究旨在评估琥珀酰磺胺噻唑对添加到含奶饮食中的叶酸表观生物利用度的影响。我们还评估了牛奶类型对巴氏杀菌（62.5摄氏度，30分钟）后叶酸相对生物利用度（生物利用度）的影响，以及叶酸（蝶酰谷氨酸）与蝶酰六聚-γ-L-谷氨酸（蝶酰谷氨酸6）的生物利用度。在叶酸耗竭（5周）后，将108只大鼠（每组6只）喂食4周含或不含20克乳固体/100克饮食的日粮，日粮中含有蝶酰谷氨酸、蝶酰谷氨酸+5克/千克琥珀酰磺胺噻唑或蝶酰谷氨酸6。使用血浆叶酸浓度和由喂食含不同水平蝶酰谷氨酸的无奶饮食的大鼠生成的标准曲线来测定叶酸生物利用度。含人乳饮食中蝶酰谷氨酸和蝶酰谷氨酸6的生物利用度是含牛或羊乳饮食的两倍（P<0.05）。将磺胺药物添加到含人乳或牛乳的饮食中会使蝶酰谷氨酸生物利用度降低一半（P<0.05）。此外，含人乳或羊乳饮食中蝶酰谷氨酸的生物利用度值不再有差异（分别为0.86和0.75），含人乳和羊乳饮食的生物利用度大于含牛乳饮食的生物利用度（0.54）（P<0.05）。牛奶的巴氏杀菌不影响叶酸生物利用度。蝶酰谷氨酸6的生物利用度为蝶酰谷氨酸的49%-71%（P<0.05）。总之，牛奶类型对肠道叶酸生物合成有不同影响，含人乳饮食中叶酸生物利用度较高部分归因于肠道叶酸生物合成增强。
