沙土鼠海马脑片标本对癫痫性去极化有抵抗，但对缺氧性去极化无抵抗。

Resistance to epileptic, but not anoxic, depolarization in the gerbil hippocampal slice preparation.

作者信息

Stittsworth J D, Lanthorn T H

机构信息

G.D. Searle & Co., Skokie, IL 60077.

出版信息

Neurosci Lett. 1994 Feb 28;168(1-2):8-10. doi: 10.1016/0304-3940(94)90403-0.

DOI:10.1016/0304-3940(94)90403-0

PMID:8028797

Abstract

The Mongolian gerbil displays spontaneous seizures and is used as a model for global ischemia. This study investigated the electrophysiological events associated with 0-Mg(2+)-induced seizures in gerbil hippocampal slices. In the rat hippocampal slice, 0-Mg2+ medium leads to rapid extracellular epileptic depolarization (ED) accompanied by long-term synaptic failure. Both evoked and spontaneous epileptiform activity was observed in the gerbil hippocampal slice after the introduction of the 0-Mg2+ aCSF. However, unlike the rat, ED was rarely observed in the gerbil hippocampal slice (2/17). When ED occurred, synaptic responses recovered (75%) within 20 min. This resistance to epileptic depolarization did not generalize to experimental ischemia-induced depolarization. Anoxia in 2 mM D-glucose produced anoxic depolarization in all gerbil hippocampal slices tested (6/6).

摘要

蒙古沙鼠会出现自发性癫痫发作，并被用作全脑缺血的模型。本研究调查了与蒙古沙鼠海马切片中0 - Mg(2+)诱导的癫痫发作相关的电生理事件。在大鼠海马切片中，0 - Mg2+培养基会导致快速的细胞外癫痫去极化（ED），并伴有长期的突触功能衰竭。在引入0 - Mg2+人工脑脊液（aCSF）后，在蒙古沙鼠海马切片中观察到了诱发性和自发性癫痫样活动。然而，与大鼠不同的是，在蒙古沙鼠海马切片中很少观察到ED（17例中有2例）。当ED发生时，突触反应在20分钟内恢复（75%）。这种对癫痫去极化的抗性并不适用于实验性缺血诱导的去极化。在2 mM D - 葡萄糖中进行缺氧处理，在所有测试的蒙古沙鼠海马切片中均产生了缺氧去极化（6/6）。