Tal-Singer R, Eisenberg R J, Valyi-Nagy T, Fraser N W, Cohen G H
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104-6003.
Virology. 1994 Aug 1;202(2):1050-3. doi: 10.1006/viro.1994.1437.
Glycoprotein D (gD) is an essential component of the herpes simplex virus (HSV) envelope. It is essential for viral penetration and for cell to cell spread of virus in vitro, and is also important for neuroinvasiveness. We investigated the contribution of N-linked oligosaccharides (N-CHO) on gD to viral pathogenesis. We used F-gD(QAA), a mutant virus derived from strain F of HSV-1. This virus contains three mutations in the gD gene which eliminate all signals for addition of N-CHO. These mutations affect the antigenic structure of gD and also lead to a small plaque phenotype. Otherwise the virus appears normal in in vitro assays. We used the mouse eye model of HSV latency to examine whether the mutations alter the phenotype of the virus in vivo. At 4 days postinfection similar amounts of F-gD(QAA) and F-gD(WT), its wild-type parent, were found in either eyes or trigeminal ganglia (TG) of infected mice. Moreover, both mutant and wild-type viruses exhibited the same ability to establish, maintain, and be reactivated from latency. We conclude that N-CHO on gD are not essential for HSV-1 pathogenesis in this model.
糖蛋白D（gD）是单纯疱疹病毒（HSV）包膜的重要组成部分。它对于病毒穿透以及病毒在体外的细胞间传播至关重要，对神经侵袭性也很重要。我们研究了gD上的N-连接寡糖（N-CHO）对病毒发病机制的作用。我们使用了F-gD(QAA)，一种源自HSV-1 F株的突变病毒。该病毒在gD基因中含有三个突变，这些突变消除了所有添加N-CHO的信号。这些突变影响gD的抗原结构，还导致小蚀斑表型。否则该病毒在体外试验中看起来正常。我们使用HSV潜伏的小鼠眼模型来检查这些突变是否会改变病毒在体内的表型。在感染后4天，在感染小鼠的双眼或三叉神经节（TG）中发现了相似数量的F-gD(QAA)及其野生型亲本F-gD(WT)。此外，突变病毒和野生型病毒在建立、维持潜伏以及从潜伏状态重新激活方面表现出相同的能力。我们得出结论，在该模型中，gD上的N-CHO对于HSV-1发病机制并非必不可少。
