Ayres M D, Howard S C, Kuzio J, Lopez-Ferber M, Possee R D
NERC Institute of Virology and Environmental Microbiology, Oxford, United Kingdom.
Virology. 1994 Aug 1;202(2):586-605. doi: 10.1006/viro.1994.1380.
The complete nucleotide sequence of the genome of clone 6 of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) has been determined. The molecule comprises 133,894 base pairs and has an overall A + T content of 59%. Our analysis suggests that the virus encodes some 154 methionine-initiated, and potentially expressed, open reading frames (ORFs) of 150 nucleotides or greater. These ORFs are distributed evenly throughout the virus genome on either strand. The ORFs are arranged as adjacent, nonoverlapping reading frames separated by short intergenic regions. Based on the primary nucleotide sequence, predictions have been made concerning the functions of certain genes, the sites for initiation of viral DNA replication, the regulation of early and late gene transcription, and factors that may affect the AcNPV gene translational efficiency. The genome sequence data confirm, with minor differences, the information obtained for other AcNPV clones. It is proposed that clone C6 is considered the archetype AcNPV for comparison purposes.
苜蓿银纹夜蛾核型多角体病毒（AcNPV）克隆6的基因组完整核苷酸序列已被测定。该分子由133,894个碱基对组成，A + T总含量为59%。我们的分析表明，该病毒编码约154个由蛋氨酸起始且可能表达的开放阅读框（ORF），其长度为150个核苷酸或更长。这些ORF均匀分布在病毒基因组的两条链上。ORF排列成相邻、不重叠的阅读框，由短的基因间隔区隔开。基于核苷酸一级序列，已对某些基因的功能、病毒DNA复制起始位点、早期和晚期基因转录调控以及可能影响AcNPV基因翻译效率的因素进行了预测。基因组序列数据证实了与其他AcNPV克隆获得的信息，仅存在微小差异。建议将克隆C6作为用于比较目的的原型AcNPV。
