Creech Kraft J, Schuh T, Juchau M R, Kimelman D
Department of Pharmacology, University of Washington, Seattle 98195.
Biochem J. 1994 Jul 1;301 ( Pt 1)(Pt 1):111-9.
Recently, the temporal and spatial distribution patterns of the retinoid receptor ligands 9-cis-retinoic acid and all-trans-retinoic acid were described in Xenopus embryos during early development [Creech Kraft, Schuh, Juchau and Kimelman (1994) Proc. Natl. Acad. Sci. U.S.A., in the press]. The present study demonstrates the presence and distribution of their likely precursors, all-trans-retinol, didehydroretinol, didehydroretinal and all-trans-retinal, as well as the occurrence of 4-oxo metabolites, in Xenopus embryos. The temporal and spatial distribution patterns of all-trans-retinol, didehydroretinol and all-trans-retinal did not coincide with that observed for 9-cis-retinoic acid but, in certain regards, were similar to the patterns delineated for all-trans-retinoic acid and all-trans-retinoyl beta-glucuronide. Evidence is presented that 9-cis-retinoic acid can be synthesized from both all-trans-retinoic acid and all-trans-retinol in Xenopus embryos, suggesting that the difference between the distributions of 9-cis-retinoic acid and the other retinoids may be caused by selective synthesis and/or protein binding of the 9-cis isomer.
最近，类视黄醇受体配体9-顺式视黄酸和全反式视黄酸在非洲爪蟾胚胎早期发育过程中的时空分布模式已有描述[克里奇·克拉夫特、舒、朱乔和基梅尔曼（1994年），《美国国家科学院院刊》，即将发表]。本研究证明了它们可能的前体全反式视黄醇、去氢视黄醇、去氢视黄醛和全反式视黄醛在非洲爪蟾胚胎中的存在和分布，以及4-氧代代谢物的出现。全反式视黄醇、去氢视黄醇和全反式视黄醛的时空分布模式与9-顺式视黄酸的不同，但在某些方面与全反式视黄酸和全反式视黄酰β-葡萄糖醛酸所描绘的模式相似。有证据表明，在非洲爪蟾胚胎中，9-顺式视黄酸可由全反式视黄酸和全反式视黄醇合成，这表明9-顺式视黄酸与其他类视黄醇分布之间的差异可能是由9-顺式异构体的选择性合成和/或蛋白质结合引起的。
