Isaji M, Momose Y, Tatsuzawa Y, Naito J
Central Research Laboratories, Kissei Pharmaceutical Co. Ltd., Hotaka, Japan.
Int Arch Allergy Immunol. 1994 Aug;104(4):340-7. doi: 10.1159/000236689.
We examined the effects of the exudate, collected from rats in which hypersensitive granulomatous inflammation was induced by methylated bovine serum albumin, on the function of inflammatory fibroblasts in culture. Addition of the exudate to the fibroblast culture induced changes in fibroblast morphology, identified by staining with antirat prolyl hydroxylase. Phase-contrast microscopy revealed that the exudate-treated fibroblasts became elongated and formed a network. Scanning electron microscopy showed that numerous long pseudopods covered the surface of the fibroblasts. Transmission electron microscopy revealed microfilament bundles with dense zones parallel to the long axis of the cell and nuclei with folds and indentations. Microfilaments were also visible in the elongated pseudopods. These features were consistent with those of myofibroblasts. The exudate also stimulated fibroblast proliferation and collagen synthesis. These findings suggest that the exudate contained certain factors that modulated fibroblast morphology, proliferation, and collagen synthesis. It is possible that putative endogenous factor(s) at the sites of inflammation modulate the development, contraction, and fibrosis of granulation tissue in hypersensitive granulomatous inflammation.
我们研究了从经甲基化牛血清白蛋白诱导产生超敏性肉芽肿性炎症的大鼠体内收集的渗出液对培养的炎性成纤维细胞功能的影响。将渗出液添加到成纤维细胞培养物中会诱导成纤维细胞形态发生变化，这可通过抗大鼠脯氨酰羟化酶染色来鉴定。相差显微镜显示，经渗出液处理的成纤维细胞变得细长并形成网络。扫描电子显微镜显示，许多长伪足覆盖在成纤维细胞表面。透射电子显微镜显示有与细胞长轴平行的微丝束和有褶皱及凹陷的细胞核。在细长的伪足中也可见微丝。这些特征与肌成纤维细胞的特征一致。渗出液还刺激成纤维细胞增殖和胶原蛋白合成。这些发现表明，渗出液中含有某些调节成纤维细胞形态、增殖和胶原蛋白合成的因子。炎症部位的假定内源性因子有可能调节超敏性肉芽肿性炎症中肉芽组织的发育、收缩和纤维化。
