Soni J L, Cox H W
Am J Trop Med Hyg. 1975 Mar;24(2):206-13.
A cold-active hemagglutinin for trypsinized human type "O" erythrocytes (CAH) from blood of chickens with acute Plasmodium gallinaceum malaria was found to be associated with 19 S and 7 S globulin fractions of malarious chicken blood, but cleavage with 2-mercaptoethanol indicated that it was primarily of the IgM class of antibody. In serologic tests CAH reacted with trypsinized erythrocytes, and anti-chicken globulin. It did not react with other of the antigens or antibodies detected in the blood of malarious chickens. When the absorbed and eluted CAH was injected into normal chickens it produced an anaphylactic-like shock and caused a 25% reduction in red blood cell counts within 48 hours. Plasma samples collected during this interval showed signs of hemolysis. Reactions of blood cells from the recipient birds with fluorescein conjugated anti-chicken globulin indicated that CAH reacted with erythrocytes. The absence of fluorescent activity 3 days after injection suggested that these erythrocytes had been removed from the circulation. When normal chickens were injected with trypsinized autologous blood cells, CAH was detected within 3 days. The agglutination test again was active at temperatures below 22 degrees C and was negative when tested at 37 degrees C. In these birds the appearance of CAH was accompanied by reductions in red blood cell counts and by hemolysis. The results of these experiments suggest that CAH was not stimulated by plasmodial parasite antigen, but rather by autoantigens, which appear to be common to heterologous animal species, and which were in some manner expressed by the presence of the intracellular parasites, or by trypsin treatment. The experiments further suggest that this autohemagglutinin was partially causal of malarial anemia. The presence of other anemia factor(s) was indicated by anemia following injection of plasma that had been absorbed free of CAH.
从患有急性鸡疟原虫疟疾的鸡血液中发现一种针对胰蛋白酶处理过的人“O”型红细胞的冷活性血凝素（CAH），它与患疟鸡血液的19S和7S球蛋白组分相关，但用2-巯基乙醇处理表明它主要属于IgM类抗体。在血清学试验中，CAH与胰蛋白酶处理过的红细胞以及抗鸡球蛋白发生反应。它不与患疟鸡血液中检测到的其他抗原或抗体发生反应。当将吸收并洗脱的CAH注射到正常鸡体内时，会产生类似过敏反应的休克，并在48小时内导致红细胞计数减少25%。在此期间采集的血浆样本显示有溶血迹象。受体鸡的血细胞与荧光素偶联的抗鸡球蛋白反应表明CAH与红细胞发生反应。注射3天后无荧光活性表明这些红细胞已从循环中清除。当给正常鸡注射胰蛋白酶处理过的自体血细胞时，3天内可检测到CAH。凝集试验在22摄氏度以下的温度下再次呈阳性，而在37摄氏度检测时为阴性。在这些鸡中，CAH的出现伴随着红细胞计数减少和溶血。这些实验结果表明，CAH不是由疟原虫抗原刺激产生的，而是由自身抗原刺激产生的，这些自身抗原似乎是异源动物物种共有的，并且以某种方式由细胞内寄生虫的存在或胰蛋白酶处理所表达。这些实验进一步表明这种自身血凝素部分导致了疟疾贫血。注射去除CAH的血浆后出现贫血表明存在其他贫血因子。
