Su M, Niu S M, Tian D P
Department of Pathology, Xian Medical University.
Zhonghua Bing Li Xue Za Zhi. 1994 Feb;23(1):34-6.
500 Tru-cut thyroid tissue specimens were analyzed by ABC immunohistochemistry technique by using monoclonal antibodies specific for activated lymphocytes, cytokines and HLA-DR antigen. The study revealed that DR antigen expression on thyrocytes was positively correlated with the total number of thyroid infiltrating cells, the percentage of Tac + cells, TLiSA1 + cells, T9+ cells, interferon-r+ cells, interleukin-2+ cells, especially I2+ cells, tumor necrosis factor+ cells and also positively correlated with serum TSH, thyroid microsomal antibody, thyroid globulin antibody levels. The DR expression on thyrocytes was most intense in autoimmune thyroiditis, with subacute thyroiditis, thyrotoxicosis with thyroiditis, Graves disease, goiter following in sequence and thyroid tumor being the weakest. Thyroxine treatment can suppress DR expression on thyrocytes. Our data suggest that the extent of DR expression on thyrocytes may be regarded as a new important index for clinical diagnosis, prognosis and evaluation of the therapeutic results.
采用抗活化淋巴细胞、细胞因子及HLA - DR抗原的单克隆抗体，运用ABC免疫组化技术对500例甲状腺Tru - cut组织标本进行分析。研究显示，甲状腺细胞上DR抗原的表达与甲状腺浸润细胞总数、Tac +细胞、TLiSA1 +细胞、T9 +细胞、干扰素 - r +细胞、白细胞介素 - 2 +细胞尤其是I2 +细胞、肿瘤坏死因子 +细胞的百分比呈正相关，也与血清促甲状腺激素、甲状腺微粒体抗体、甲状腺球蛋白抗体水平呈正相关。甲状腺细胞上的DR表达在自身免疫性甲状腺炎中最为强烈，亚急性甲状腺炎、伴有甲状腺炎的甲状腺毒症、格雷夫斯病、甲状腺肿依次次之，甲状腺肿瘤最弱。甲状腺素治疗可抑制甲状腺细胞上的DR表达。我们的数据表明，甲状腺细胞上DR表达的程度可被视为临床诊断、预后及治疗效果评估的一项新的重要指标。
