大肠杆菌三个乳糖操纵基因的质量和位置决定了阻遏效率。
Quality and position of the three lac operators of E. coli define efficiency of repression.
作者信息
Oehler S, Amouyal M, Kolkhof P, von Wilcken-Bergmann B, Müller-Hill B
机构信息
Institut für Genetik der Universität zu Köln, Germany.
出版信息
EMBO J. 1994 Jul 15;13(14):3348-55. doi: 10.1002/j.1460-2075.1994.tb06637.x.
Abstract
Repression of the lac promoter may be achieved in two different ways: either by interference with the action of RNA polymerase or by interference with CAP activation. We investigated cooperative repression of the Escherichia coli lac operon by systematic conversion of its three natural operators (O1, O2 and O3) on the chromosome. We find that cooperative repression by tetrameric Lac repressor increases with both quality and proximity of the interacting operators. A short distance of 92 bp allows effective repression by two very weak operators (O3, O3). The cooperativity of lac operators is discussed in terms of a local increase of repressor concentration. This increase in concentration depends on flexible DNA which allows loop formation.
摘要
对乳糖启动子的阻遏可通过两种不同方式实现
要么通过干扰RNA聚合酶的作用，要么通过干扰CAP激活。我们通过对大肠杆菌乳糖操纵子染色体上的三个天然操纵基因（O1、O2和O3）进行系统转换，研究了其协同阻遏作用。我们发现，四聚体乳糖阻遏物的协同阻遏作用随着相互作用的操纵基因的质量和接近程度而增加。92个碱基对的短距离允许两个非常弱的操纵基因（O3、O3）进行有效阻遏。从阻遏物浓度的局部增加角度讨论了乳糖操纵基因的协同性。这种浓度增加依赖于允许形成环的柔性DNA。
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