Singh N, Arora V K, Ramam M, Tickoo S K, Bhatia A
Department of Pathology, University College of Medical Sciences, Delhi, India.
Int J Lepr Other Mycobact Dis. 1994 Jun;62(2):263-7.
Forty biopsies of granulomatous dermatoses, 12 of which were tuberculoid leprosy (TL), were studied for patterns of nerve twig distribution using an immunoperoxidase technique for S-100 protein. Four distinct patterns of nerve twigs were identified: 1) within granulomas, 2) between granulomas, 3) within and between granulomas, and 4) undetectable nerve twigs in an adequate biopsy. Pattern 4 was seen exclusively in TL (p < 0.05). The other patterns occurred in nonleprosy dermatoses as well, suggesting that pattern 4 is the best indicator toward a diagnosis of TL. The granules of mycetoma and Mycobacterium leprae also stained positively with the S-100 stain.
对40例肉芽肿性皮肤病活检标本进行研究，其中12例为结核样麻风（TL），采用免疫过氧化物酶法检测S-100蛋白，观察神经小分支的分布模式。识别出4种不同的神经小分支模式：1）在肉芽肿内；2）在肉芽肿之间；3）在肉芽肿内及肉芽肿之间；4）在足够的活检标本中未检测到神经小分支。模式4仅见于结核样麻风（p<0.05）。其他模式也见于非麻风性皮肤病，提示模式4是结核样麻风诊断的最佳指标。足菌肿颗粒和麻风分枝杆菌的S-100染色也呈阳性。
