Ray R, Panda C K, Chakraborty B K, Mukherji S, Chaudhury K, Roychoudhury J
Department of Biochemistry, Chittaranjan National Cancer Institute, Calcutta, India.
Mol Cell Biochem. 1994 Feb 9;131(1):67-73. doi: 10.1007/BF01075726.
Partial hepatectomy (P.H.) induces a partially synchronized growth response of liver under normal regulation of growth. In this phase changes in cellular morphology, radial distribution pattern of cells and other biological as well as major biochemical changes are well documented [24]. Here, we have shown that the cellular content of UsnRNAs altered during this proliferative phase as well. The level of spliceosomal UsnRNAs (U1, U2, U4-U6) gradually decreased by 30-50% upto 48 hrs of P.H. followed by gradual increase to reach the normal level within one month of P.H. The U3 snRNA level on the other hand, was nearly equal to that in normal liver at 48 hrs of P.H. but in 24 and 72 hrs of P.H. its level was high (4 fold) in contrast to that in other UsnRNAs. Thus, it is clear from our data that the level of all the six UsnRNAs decreased during 48 hrs of P.H. compared to that after first 24 hrs. This has been correlated in the kinetics of UsnRNAs' synthesis (in terms of labelling) in isolated hepatocytes, where the rate of labelling of all the six UsnRNAs increased 20-30% in 24 hrs regenerating hepatocytes (R.H.) followed by sharp decrease by 30-50% within next 24 hrs, compared to that in the normal hepatocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
部分肝切除术（P.H.）在正常生长调节下诱导肝脏产生部分同步的生长反应。在这个阶段，细胞形态的变化、细胞的径向分布模式以及其他生物学和主要生化变化都有充分的记录[24]。在这里，我们已经表明，在这个增殖阶段，UsnRNAs的细胞含量也发生了改变。剪接体UsnRNAs（U1、U2、U4 - U6）的水平在部分肝切除术后48小时内逐渐下降30 - 50%，随后逐渐上升，在部分肝切除术后一个月内恢复到正常水平。另一方面，U3 snRNA水平在部分肝切除术后48小时时与正常肝脏几乎相等，但在部分肝切除术后24小时和72小时时，其水平较高（4倍），与其他UsnRNAs相反。因此，从我们的数据可以清楚地看出，与最初24小时后相比，在部分肝切除术后48小时内所有六种UsnRNAs的水平都下降了。这与分离的肝细胞中UsnRNAs合成的动力学（就标记而言）相关，其中在24小时再生肝细胞（R.H.）中所有六种UsnRNAs的标记率增加了20 - 30%，随后在接下来的24小时内急剧下降30 - 50%，与正常肝细胞相比。（摘要截断于250字）
