花生四烯酸和类二十烷酸可能参与了海葵（腔肠动物门；水螅纲）的变态过程。

Possible involvement of arachidonic acid and eicosanoids in metamorphic events in Hydractinia echinata (Coelenterata; Hydrozoa).

作者信息

Leitz T, Beck H, Stephan M, Lehmann W D, De Petrocellis L, Di Marzo V

机构信息

Zoologisches Institut der Universität, Heidelberg, Germany.

出版信息

J Exp Zool. 1994 Aug 1;269(5):422-31. doi: 10.1002/jez.1402690505.

DOI:10.1002/jez.1402690505

PMID:8057074

Abstract

Upon induction of metamorphosis, larvae of the marine hydroid Hydractinia echinata release [14C]-arachidonic acid from previously labeled endogenous sources. The lipoxygenase inhibitors nordihydroguaiaretic acid and 5,8,11,14-eicosatetraynoic acid inhibited metamorphosis induced by Cs+ and 1,2-sn-dioctanoylglycerol, whereas the inhibitors of cyclooxygenase, indomethacin, and acetylsalicylic acid were ineffective, suggesting a role for lipoxygenase metabolites of arachidonic acid in induction of metamorphosis. Lipoxygenase products in Hydractinia echinata were isolated and identified by gas chromatography/mass spectrometry. 8- and 12-HETE were the most abundant metabolites. In cytosolic fractions from larvae activity of an arachidonic acid metabolizing enzyme, presumably a lipoxygenase, was found. The metabolic product was identified by 1H-NMR and chiral phase HPLC as 8(R)-HETE. Its production was strongly inhibited by NDGA, but not by indomethacin.

摘要

在诱导变态时，海洋水螅Hydractinia echinata的幼虫会从先前标记的内源性来源释放[14C] - 花生四烯酸。脂氧合酶抑制剂去甲二氢愈创木酸和5,8,11,14 - 二十碳四烯酸抑制了由Cs +和1,2 - 二辛酰基 - sn - 甘油诱导的变态，而环氧化酶抑制剂吲哚美辛和乙酰水杨酸则无效，这表明花生四烯酸的脂氧合酶代谢产物在变态诱导中起作用。通过气相色谱/质谱法分离并鉴定了Hydractinia echinata中的脂氧合酶产物。8 - 和12 - HETE是最丰富的代谢产物。在幼虫的胞质组分中发现了一种花生四烯酸代谢酶（可能是脂氧合酶）的活性。通过1H - NMR和手性相HPLC鉴定代谢产物为8(R) - HETE。其产生受到NDGA的强烈抑制，但不受吲哚美辛的抑制。