Behling R G, Wong A C
Department of Food Microbiology, University of Wisconsin-Madison 53706.
Int J Food Microbiol. 1994 Apr;22(1):1-9. doi: 10.1016/0168-1605(94)90002-7.
Oral inoculation of lactose utilizing cecal bacteria plus 2.5% lactose treatments were tested in young chicks for protective efficacy against infection by Salmonella enteritidis. One-day-old chicks were treated with cecal bacteria upon arrival and challenged orally on day 3 with 10(4)-10(6) cfu S. enteritidis. A single culture identified as Escherichia coli O75:H10 was found significantly more protective than all other isolates tested. This isolate excreted a metabolite(s) in vitro that was inhibitory towards the growth of S. enteritidis. The results of this study indicate that discovery of protective strains can be facilitated by screening isolates in vitro for lactose utilization and growth inhibition of S. enteritidis before administration of treatment.
对雏鸡进行了口服利用乳糖的盲肠细菌加2.5%乳糖处理的试验，以测试其对肠炎沙门氏菌感染的保护效果。一日龄雏鸡到达后用盲肠细菌处理，并在第3天口服10(4)-10(6) cfu肠炎沙门氏菌进行攻毒。发现一种鉴定为大肠杆菌O75:H10的单一培养物比所有其他测试分离株具有显著更高的保护作用。该分离株在体外分泌一种对肠炎沙门氏菌生长有抑制作用的代谢物。本研究结果表明，在进行治疗前，通过体外筛选分离株对乳糖的利用情况以及对肠炎沙门氏菌生长的抑制作用，有助于发现保护性菌株。
