转苏云金芽孢杆菌毒素基因的转基因黑杨植株的抗虫性
Insect tolerance of transgenic Populus nigra plants transformed with Bacillus thuringiensis toxin gene.
作者信息
Tian Y, Li T, Mang K, Han Y, Li L, Wang X, Lu M, Dai L, Han Y, Yan J
机构信息
Institute of Microbiology, Chinese Academy of Sciences, Beijing.
出版信息
Chin J Biotechnol. 1993;9(4):219-27.
Leaves and stem segments of Populus nigra were transformed with A. tumefaciens LBA4404 harboring a binary vector containing chimeric genes of NPT and 35S-omega-B.t. toxin-Nos. Nineteen regenerated kanamycin resistant plants were analyzed by DNA hybridization, out of which 10 were shown to be the candidates of transgenic plants. Insect tolerance tests showed that the transgenic plants were toxic to two lepidopteran pests, Lymantria dispar L. and Apocheimia cinerarius Erschoff. Based on the results of Southern blot of PCR products and the cluster analysis of their growth and insect resistance, three independent transgenic plants were selected, which were then propagated in the nursery field. This field test is currently in progress.
用携带含有新霉素磷酸转移酶（NPT）和35S-ω-苏云金芽孢杆菌毒素-Nos嵌合基因的二元载体的根癌农杆菌LBA4404转化黑杨的叶片和茎段。通过DNA杂交分析了19株再生的卡那霉素抗性植株，其中10株被证明是转基因植株的候选者。昆虫耐受性测试表明，转基因植株对两种鳞翅目害虫舞毒蛾和杨扇舟蛾有毒性。根据PCR产物的Southern印迹结果及其生长和抗虫性的聚类分析，选择了3株独立的转基因植株，然后在苗圃中繁殖。目前正在进行该田间试验。
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