Blasi E, Pitzurra L, Puliti M, Mazzolla R, Barluzzi R, Saleppico S, Bistoni F
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Cell Immunol. 1994 Sep;157(2):501-9. doi: 10.1006/cimm.1994.1245.
Using an in vitro experimental model, we have recently demonstrated that Candida albicans in its hyphal form (H-Candida), similarly to lipopolysaccharide (LPS), enhances tumor necrosis factor (TNF) secretory response in the cloned macrophage (M phi) population ANA-1. Here we show that H-Candida and LPS each differ in their requirements for intact protein kinase functions, susceptibility to 0.4-microns micropore-size membranes, and sensitivity to polymyxin B. These results, together with the synergistic effect occurring between H-Candida and LPS in inducing TNF response, indicate the existence of different receptor(s) and/or signal-transduction pathway(s) through which the two stimuli act.
利用体外实验模型，我们最近证明，白色念珠菌的菌丝形态（H-念珠菌）与脂多糖（LPS）类似，可增强克隆巨噬细胞（M phi）群体ANA-1中的肿瘤坏死因子（TNF）分泌反应。在此我们表明，H-念珠菌和LPS在对完整蛋白激酶功能的需求、对0.4微米微孔尺寸膜的敏感性以及对多粘菌素B的敏感性方面各有不同。这些结果，连同H-念珠菌和LPS在诱导TNF反应中产生的协同效应，表明存在两种刺激作用所通过的不同受体和/或信号转导途径。
