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肉类发酵中的固定化细胞。

Immobilized cells in meat fermentation.

作者信息

McLoughlin A J, Champagne C P

机构信息

Department of Industrial Microbiology, University College Dublin, Ireland.

出版信息

Crit Rev Biotechnol. 1994;14(2):179-92. doi: 10.3109/07388559409086967.

Abstract

The immobilization of microbial cells can contribute to fermented meat technology at two basic levels. First, the solid/semisolid nature (low available water) of the substrate restricts the mobility of cells and results in spatial organizations based on "natural immobilization" within the fermentation matrix. The microniches formed influence the fermentation biochemistry through mass transfer limitations and the subsequent development and activity of the microflora. This form of immobilization controls the nature of competition between subpopulations within the microflora and ultimately exerts an effect on the ecological competence (ability to survive and compete) of the various cultures present. Second, immobilized cell technology (ICT) can be used to enhance the ecological competence of starter cultures added to initiate the fermentation. Immobilization matrices such as alginate can provide microniches or microenvironments that protect the culture during freezing or lyophilization, during subsequent rehydration, and when in competition with indigenous microflora. The regulated release of cells from the microenvironments can also contribute to competitive ability. The regulation of both immobilization processes can result in enhanced fermentation activity.

摘要

微生物细胞的固定化可在两个基本层面上推动发酵肉制品技术的发展。首先,底物的固体/半固体性质(低有效水分)限制了细胞的移动性,并导致基于发酵基质内“自然固定化”的空间组织形式。所形成的微生态位通过传质限制以及随后微生物群落的发育和活性影响发酵生物化学过程。这种固定化形式控制着微生物群落内亚群体之间竞争的性质,并最终对存在的各种培养物的生态竞争力(生存和竞争能力)产生影响。其次,固定化细胞技术(ICT)可用于提高添加的起始培养物的生态竞争力,以启动发酵过程。诸如藻酸盐之类的固定化基质可提供微生态位或微环境,在冷冻或冻干期间、随后的复水过程中以及与本地微生物群落竞争时保护培养物。细胞从微环境中的受控释放也有助于提高竞争能力。对这两种固定化过程的调控均可提高发酵活性。

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