人源癌细胞A-431中的几种钠传导通道类型。

Several types of sodium-conducting channel in human carcinoma A-431 cells.

作者信息

Vedernikova E A, Mozhayeva G N

机构信息

Institute of Cytology, Russian Academy of Sciences, St. Petersburg.

出版信息

Biochim Biophys Acta. 1994 Aug 24;1194(1):171-5. doi: 10.1016/0005-2736(94)90217-8.

DOI:10.1016/0005-2736(94)90217-8

PMID:8075133

Abstract

Patch clamp method in outside-out configuration was used to search for cation channels which possibly mediate sodium influx through plasma membrane in A-431 carcinoma cells. We found four types of nonvoltage-gated Na-conducting channel. The first of 9-10 pS conductance (145 mM Na+, 30 degrees C) seems to be Na-selective; three others were characterized with conductance values of 24, 35 and 65 pS and lower selectivity among cations. Na-selective channels (9-10 pS) were not blocked by tetrodotoxin (1 microM). External application of amiloride (0.1-2 mM) resulted in a reversible inhibition of single currents through Na-selective channels.

摘要

采用外向式膜片钳技术在A - 431癌细胞中寻找可能介导钠通过质膜内流的阳离子通道。我们发现了四种非电压门控的钠传导通道。第一种通道在9 - 10 pS电导（145 mM钠，30摄氏度）时似乎对钠具有选择性；另外三种通道的电导值分别为24、35和65 pS，对阳离子的选择性较低。钠选择性通道（9 - 10 pS）不受河豚毒素（1 microM）的阻断。外用阿米洛利（0.1 - 2 mM）可导致通过钠选择性通道的单电流可逆性抑制。