Taurine and beta-alanine transport in an established human kidney cell line derived from the proximal tubule.

The transport mechanisms of taurine and beta-alanine by an immortalized human embryonic kidney epithelial cell line (IHKE) were examined. The uptake of these beta-amino acids was characterized by two Na(+)-dependent transport components, whereas an inwardly directed H(+)-gradient only stimulated amino acid influx to a small extent and in the absence of sodium. Competition experiments revealed that taurine and beta-alanine drastically reduced the uptake of one another by the high-affinity Na(+)-dependent transport system. However, some alpha-amino acids could also compete with the beta-amino acids, but with a low affinity. Examinations of the effect of different anions on the Na(+)-dependent uptake of taurine at a low amino acid concentration (240 nM) revealed a specific requirement for Cl-, whereas Cl- had no measurable effect at a higher concentration (1.0 mM) of taurine. In addition, activation of taurine transport as a function of Na+ and Cl- concentration indicated a probable coupling ratio of 3 Na+/1 Cl-/1 taurine for the high-affinity carrier. Finally, cellular regulation of taurine transport was indicated by the finding that pretreatment with taurine containing media decreased the activity of the taurine transporter(s).