采用玻碳工作电极检测器的高效液相色谱法测定生物样品中的谷胱甘肽和半胱氨酸
Glutathione and cysteine measurement in biological samples by HPLC with a glassy carbon working detector.
作者信息
Carvalho F D, Remião F, Vale P, Timbrell J A, Bastos M L, Ferreira M A
机构信息
Laboratory of Toxicology, Faculty of Pharmacy, University of Oporto, Portugal.
出版信息
Biomed Chromatogr. 1994 May-Jun;8(3):134-6. doi: 10.1002/bmc.1130080308.
An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 +/- 4.15, 4.50 +/- 0.65, and 0.19 +/- 0.12 mM (means +/- SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N-ethylmaleimide.
开发了一种使用玻碳工作电极检测器的高效液相色谱法，用于测定生物样品中的半胱氨酸和还原型谷胱甘肽（GSH）。在用谷胱甘肽还原酶还原后，还测定了氧化型谷胱甘肽（GSSG）。本研究在人血浆中进行。半胱氨酸和GSH标准曲线在生理范围内呈线性，检测限分别为22.0皮摩尔和6.0皮摩尔。所测得的血浆中半胱氨酸、GSH和GSSG的结果分别为43.92±4.15、4.50±0.65和0.19±0.12毫摩尔（平均值±标准误）。用N-乙基马来酰亚胺处理后半胱氨酸和还原型谷胱甘肽的峰特异性通过它们的消失得以证实。
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