Asada H, Uyemura K, Shirao T
Department of Physiology, School of Medicine, Keio University, Tokyo, Japan.
J Neurosci Res. 1994 Jun 1;38(2):149-59. doi: 10.1002/jnr.490380205.
Drebrins are developmentally regulated actin-binding proteins. In this study, we analyzed subcellular distribution of drebrin E in neuroblastoma cells (SH-SY5Y) in culture, especially in terms of its relationship to actin filaments. In undifferentiated cells, drebrin E was scattered as flocculus small dots along the stress fibers and also accumulated at adhesion plaques. In parallel with the neuronal differentiation following retinoic acid treatment, drebrin E was accumulated, accompanying filamentous (F) actin, in the submembranous cortical cytoplasm. Similar submembranous localization of drebrins was observed in primary cultured neurons. In the presence of drebrin E F-actin was more stable against cytochalasin D than F-actin lacking drebrin E. These results suggest that drebrin E plays a role in neuronal morphological differentiation by changing its subcellular localization with stabilized F-actin.
双调蛋白是受发育调控的肌动蛋白结合蛋白。在本研究中，我们分析了双调蛋白E在培养的神经母细胞瘤细胞（SH-SY5Y）中的亚细胞分布，特别是其与肌动蛋白丝的关系。在未分化细胞中，双调蛋白E沿应力纤维呈絮状小点分散分布，并且也聚集在黏着斑处。随着视黄酸处理后神经元的分化，双调蛋白E与丝状（F）肌动蛋白一起在膜下皮质细胞质中积累。在原代培养的神经元中也观察到双调蛋白类似的膜下定位。在存在双调蛋白E的情况下，F-肌动蛋白比缺乏双调蛋白E的F-肌动蛋白对细胞松弛素D更稳定。这些结果表明，双调蛋白E通过改变其亚细胞定位并稳定F-肌动蛋白，在神经元形态分化中发挥作用。
