Basu N, Kole L, Ghosh A, Das P K
Department of Molecular Cell Biology, Indian Institute of Chemical Biology, Calcutta.
Mol Cell Biochem. 1994 Mar 16;132(1):1-6. doi: 10.1007/BF00925668.
A rabbit polyclonal antibody to crude soluble antigen of Leishmania donovani promastigotes recognized a determinant expressed on the surface membrane of mouse peritoneal macrophages and human monocyte derived macrophages infected in vitro. The determinant was recognized on infected macrophage surface only when F(ab')2 fragments of anti-leishmanial antiserum was employed in immunofluorescence. F(ab')2 fragments of human patient sera also could recognize the determinant. The expression of this antigen was not stage-specific for the parasite. Immunochemical analyses revealed this antigen to be of 51 kDa protein. Specific leaching of membrane proteins by trypsin showed three bands of expressed antigens of 26, 11 and 10 kDa, which in all likelihood might be arising from the 51 kDa antigen. The antigen was not expressed until 12 h of post infection, reached a maximum level at 24 h and thereafter attained a steady state level as studied upto 96 h of post infection. This type of antigen might have a great potential in immunodiagnostics and site-specific drug targeting.
一种针对杜氏利什曼原虫前鞭毛体粗可溶性抗原的兔多克隆抗体，识别在体外感染的小鼠腹腔巨噬细胞和人单核细胞衍生巨噬细胞表面膜上表达的一个决定簇。仅当在免疫荧光中使用抗利什曼原虫抗血清的F(ab')2片段时，才能在感染的巨噬细胞表面识别该决定簇。人类患者血清的F(ab')2片段也能够识别该决定簇。这种抗原的表达对寄生虫而言并非阶段特异性的。免疫化学分析表明该抗原为51 kDa的蛋白质。用胰蛋白酶对膜蛋白进行特异性洗脱显示出三条表达抗原带，分别为26 kDa、11 kDa和10 kDa，它们很可能源自51 kDa的抗原。感染后12小时之前该抗原未表达，在24小时达到最高水平，此后直至感染后96小时研究期间达到稳定状态水平。这类抗原在免疫诊断和位点特异性药物靶向方面可能具有巨大潜力。
