盘基网柄菌细胞中糖基磷脂酰肌醇锚定细胞粘附分子的组成型过表达。

Constitutive overexpression of a GPI-anchored cell adhesion molecule in Dictyostelium discoideum cells.

作者信息

Silvestri M L, Pivi A M, Klein C, Pauly P C, DaSilva A M

机构信息

Departamento de Bioquímica, Universidade de São Paulo, Brasil.

出版信息

Braz J Med Biol Res. 1994 Feb;27(2):427-30.

PMID:8081260

Abstract

gp80 is the best characterized cell adhesion molecule of Dictyostelium discoideum. It has a glycolipid anchor that, although it shows resistance to exogenous GPI-PLC, is sensitive to an endogenous hydrolyzing activity. Preliminary data suggest that acylation on the inositol ring is not the basis for this resistance. In order to investigate the features of glycolipid anchoring during Dictyostelium development we have obtained stable transformants that overexpress gp80 during its whole life cycle.

摘要

gp80是盘基网柄菌中特征最明确的细胞粘附分子。它有一个糖脂锚定物，尽管它对外源GPI-PLC有抗性，但对内源水解活性敏感。初步数据表明，肌醇环上的酰化不是这种抗性的基础。为了研究盘基网柄菌发育过程中糖脂锚定的特征，我们获得了在其整个生命周期中过表达gp80的稳定转化体。