Iyer V, Fernandes T, Apte S K
Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay, India.
J Bacteriol. 1994 Sep;176(18):5868-70. doi: 10.1128/jb.176.18.5868-5870.1994.
The molecular basis of tolerance to osmotic stress was investigated with a cyanobacterium, Anabaena sp. strain L-31. The inherent osmotolerance of this strain (50% growth inhibition at 350 mM sucrose) was enhanced by adaptation with 100 mM sucrose for 30 min. Addition of 10 mM KNO3 during growth also conferred significant osmoprotection, but addition of 3 mM NH4Cl did not. Exposure of cells to 350 mM sucrose induced the expression of at least 12 osmotic-stress-induced proteins (OSPs) within 30 min, in the molecular mass range of 11.5 to 84 kDa. Exposure of cells to 100 mM sucrose or to 10 mM nitrate also induced all the OSPs, but addition of ammonium did not. The observed correspondence between the presence of OSPs and osmotolerance strongly suggests a role for OSPs in osmotolerance of Anabaena sp. strain L-31.
利用蓝细菌鱼腥藻（Anabaena sp.）菌株L - 31研究了对渗透胁迫耐受性的分子基础。该菌株的固有耐渗透压性（在350 mM蔗糖下生长抑制50%）通过用100 mM蔗糖适应30分钟而增强。在生长过程中添加10 mM KNO₃也赋予了显著的渗透保护作用，但添加3 mM NH₄Cl则没有。细胞暴露于350 mM蔗糖中在30分钟内诱导了至少12种渗透胁迫诱导蛋白（OSP）的表达，分子量范围为11.5至84 kDa。细胞暴露于100 mM蔗糖或10 mM硝酸盐中也诱导了所有的OSP，但添加铵则没有。观察到的OSP的存在与耐渗透压性之间的对应关系强烈表明OSP在鱼腥藻菌株L - 31的耐渗透压性中起作用。
