蝾螈嗅觉受体细胞中第二信使对钙离子浓度的调节

Regulation of Ca2+ concentration by second messengers in newt olfactory receptor cell.

作者信息

Nakamura T, Tsuru K, Miyamoto S

机构信息

Department of Applied Physics and Chemistry, University of Electro-Communications, Tokyo, Japan.

出版信息

Neurosci Lett. 1994 Apr 25;171(1-2):197-200. doi: 10.1016/0304-3940(94)90638-6.

DOI:10.1016/0304-3940(94)90638-6

PMID:8084489

Abstract

The role of IP3 in olfactory transduction is controversial. This issue was addressed by measuring intracellular Ca2+ concentration under voltage clamp in newt olfactory receptor cells. Odorant stimulation and dialysis of cells with cyclic AMP (cAMP) caused an increase in intracellular Ca2+ concentration that originated in the cilia, as expected from previous works. However, dialysis with IP3 caused a slower rise in Ca2+ concentration that originated in the cell body. Our results suggest that IP3 influences transduction by regulating the intracellular Ca2+ concentration, rather than by carrying the transduction current.

摘要

肌醇三磷酸（IP3）在嗅觉转导中的作用存在争议。通过在蝾螈嗅觉受体细胞中进行电压钳制测量细胞内钙离子浓度来探讨这一问题。正如先前研究预期的那样，气味刺激以及用环磷酸腺苷（cAMP）对细胞进行透析会导致起源于纤毛的细胞内钙离子浓度升高。然而，用IP3进行透析会使起源于细胞体的钙离子浓度上升更为缓慢。我们的结果表明，IP3通过调节细胞内钙离子浓度而非传导转导电流来影响转导过程。