牛弹性蛋白基因中存在功能性TATA盒（ATAAAA）序列的证据。

Evidence for the presence of a functional TATA box (ATAAAA) sequence in the gene for bovine elastin.

作者信息

Manohar A, Anwar R A

机构信息

Department of Biochemistry, University of Toronto, Ontario, Canada.

出版信息

Biochim Biophys Acta. 1994 Sep 13;1219(1):233-6. doi: 10.1016/0167-4781(94)90279-8.

DOI:10.1016/0167-4781(94)90279-8

PMID:8086470

Abstract

The wild type sequence (wt; ATAAAA) was mutated and the effects of the mutants were determined by assaying the expression of the chloramphenicol acetyltransferase (CAT)-encoding gene (cat) cloned downstream. The negative mutant (neg; ACGAAA) practically abolished the activities of the 129 bp and the 416 bp elastin promoters in NIH 3T3 and neonatal rat aortic smooth muscle cells, respectively. However, when the positive mutant (pos; TATAAAA) was assayed in parallel experiments, there was enhancement of activity. The TATA box-binding protein (TBP) was shown to bind to the ATAAAA sequence and the retardation of the band was abolished by competition assay using unlabeled wt and pos sequences, but not by the neg mutant. These results provide evidence for the presence of a functional TATA box in the gene for elastin.

摘要

野生型序列（wt；ATAAAA）发生了突变，并通过检测克隆在下游的氯霉素乙酰转移酶（CAT）编码基因（cat）的表达来确定突变体的作用。阴性突变体（neg；ACGAAA）分别在NIH 3T3细胞和新生大鼠主动脉平滑肌细胞中几乎完全消除了129 bp和416 bp弹性蛋白启动子的活性。然而，在平行实验中检测阳性突变体（pos；TATAAAA）时，活性增强。结果表明，TATA盒结合蛋白（TBP）与ATAAAA序列结合，并且使用未标记的wt和pos序列进行竞争分析可消除条带的阻滞，但neg突变体则不能。这些结果为弹性蛋白基因中存在功能性TATA盒提供了证据。