Cui X, Tateno H, Hayata I, Sato K, Kamiguchi Y
Sichuan Family Planning Research Institute, Chengdu, China.
Jpn J Hum Genet. 1994 Jun;39(2):255-8. doi: 10.1007/BF01876846.
A method of chromosome painting on human sperm chromosomes using fluorescent in situ hybridization (FISH) is introduced. Sperm chromosome slides were prepared after in vitro fertilization of hamster eggs with human spermatozoa. The slides were treated by RNase A before FISH. Chromosome 4 was clearly and specifically painted in a majority of sperm-derived metaphase plates after an application of whole chromosome painting DNA probes of this chromosome. This is the first report of successful painting on human sperm chromosomes.
介绍了一种使用荧光原位杂交（FISH）对人类精子染色体进行染色体涂染的方法。用人精子对仓鼠卵进行体外受精后制备精子染色体玻片。在FISH之前，玻片用核糖核酸酶A处理。应用该染色体的全染色体涂染DNA探针后，在大多数精子来源的中期板中，4号染色体被清晰且特异性地涂染。这是关于人类精子染色体成功涂染的首次报道。
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