Kumagai M
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1994 Jul;46(7):555-61.
To reveal the usefulness of Ca ionophore A23187-induced acrosome reactions (AR) in evaluating sperm fertilizing ability, the relationship between the percentage of AR and the results of the hamster test was examined in 65 men. Acrosome reacted spermatozoa were classified as type A (with FITC-PSA stain at the equatorial segment) or type B (without stain) and as alive or dead by staining with Hoechst 33258. The main results were as follows. 1. During the insemination period of 3 hours with zona-free hamster oocytes, sperm motility and the percentage of live type A spermatozoa (%AR) decreased significantly (p < 0.01). 2. Among the parameters examined, the %AR in the inseminating suspension exhibited the closest correlation with the sperm penetration rate (SPR) in the hamster test. 3. Among the 27 men with a %AR of less than 5%, 20 men (74.1) had SPRs less than 30%, indicating abnormal sperm fertilizing ability. In contrast, among the 19 men with %AR of 10% or more, 16 men (84.2%) had a SPR of 100%. These results suggest that the ionophore-induced acrosome reaction (AR) well predicts sperm fertilizing ability. We propose new criteria using %AR as follows: abnormal, < 5%; borderline, 5-10%; normal, > or = 10%.
为揭示钙离子载体A23187诱导的顶体反应（AR）在评估精子受精能力方面的实用性，我们对65名男性进行了研究，检测了AR百分比与仓鼠试验结果之间的关系。顶体反应的精子被分为A 型（赤道段有FITC-PSA染色）或B型（无染色），并用Hoechst 33258染色区分死活。主要结果如下：1. 在与无透明带仓鼠卵母细胞受精的3小时内，精子活力和活A型精子百分比（%AR）显著下降（p<0.01）。2. 在检测的各项参数中，授精悬液中的%AR与仓鼠试验中的精子穿透率（SPR）相关性最强。3. 在27名%AR低于5%的男性中，20名男性（74.1%）的SPR低于30%，表明精子受精能力异常。相比之下，在19名%AR为10%或更高的男性中，16名男性（84.2%）的SPR为100%。这些结果表明，离子载体诱导的顶体反应（AR）能很好地预测精子受精能力。我们提出以下以%AR为依据的新标准：异常，<5%；临界，5-10%；正常，>或 = 10%。
