离体大鼠肝细胞对去唾液酸胎球蛋白的摄取与降解
Uptake and degradation of asialo-fetuin by isolated rat hepatocytes.
作者信息
Tolleshaug H, Berg T
出版信息
Acta Biol Med Ger. 1977;36(11-12):1753-62.
125I-labelled asialo-fetuin was taken up by isolated rat hepatocytes by a saturable process. Half maximum uptake was seen at about 3 . 10(-8) M asialo-fetuin. Rate of uptake of asialo-fetuin exceeded rate of degradation at all concentrations of asialo-fetuin tested. Degradation of asialo-fetuin, as indicated by release of acid-soluble radioactivity from the cells, was inhibited by NH4Cl and chloroquine. The intracellular distribution of labelled asialo-fetuin was studied by differential and density gradient centrifuging. The distribution curves for radioactivity indicated that asialo-fetuin was present in lysosomes about 1 h after the uptake had started. Chloroquine and ammonium ions seemed to inhibit the uptake of asialo-fetuin into the lysosomes, possibly by interfering with the fusion between phagosomes and lysosomes.
125I标记的去唾液酸胎球蛋白可通过可饱和过程被分离的大鼠肝细胞摄取。在约3×10⁻⁸ M去唾液酸胎球蛋白浓度时出现半数最大摄取量。在所测试的所有去唾液酸胎球蛋白浓度下，其摄取速率均超过降解速率。细胞中酸溶性放射性物质的释放表明，NH₄Cl和氯喹可抑制去唾液酸胎球蛋白的降解。通过差速离心和密度梯度离心研究了标记的去唾液酸胎球蛋白在细胞内的分布。放射性分布曲线表明，摄取开始约1小时后，去唾液酸胎球蛋白存在于溶酶体中。氯喹和铵离子似乎可抑制去唾液酸胎球蛋白进入溶酶体，可能是通过干扰吞噬体与溶酶体之间的融合。
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