Roessner A, van Husen N, Pauls E, Gerlach U, Themann H
Virchows Arch A Pathol Anat Histol. 1975 Jun 23;367(1):15-26. doi: 10.1007/BF00430769.
Reports on correlations between the activity of so-called "marker enzymes of cholestasis" in serum and the ultrastructural changes of the liver are rare. Therefore studies of ultrastructural changes were carried out in 40 patients with intrahepatic cholestasis. In the patients' serum activity of alkaline phosphatase, bile duct alkaline phosphatase, leucine-aminopeptidase (LAP), and 5'-nucleotidase (5'-Nu) as well as the concentration of bilirubin were determined. The results showed a significant correlation between the morphometry of the bile canaliculi and the serum activity of LAP and 5'-Nu. In patients with elevated LAP, an enlargement of the bile canaliculi could be proved. An increased serum activity of 5'-Nu correlated with a higher incidence of bile canaliculi in the ultrastructural picture. The results suggest an investigation of the ultrastructure of bile canaliculi and the determination of marker enzymes of cholestasis in the serum may both contribute to the assessment of cholestatic liver disease.
关于血清中所谓“胆汁淤积标志物酶”的活性与肝脏超微结构变化之间相关性的报道很少。因此，对40例肝内胆汁淤积患者进行了超微结构变化的研究。测定了患者血清中碱性磷酸酶、胆管碱性磷酸酶、亮氨酸氨基肽酶（LAP）和5'-核苷酸酶（5'-Nu）的活性以及胆红素浓度。结果显示胆小管形态计量学与LAP和5'-Nu的血清活性之间存在显著相关性。在LAP升高的患者中，可以证实胆小管增大。5'-Nu血清活性增加与超微结构图像中胆小管发生率较高相关。结果表明，对胆小管超微结构的研究和血清中胆汁淤积标志物酶的测定都可能有助于评估胆汁淤积性肝病。
