Buchko G W, Cadet J, Ravanat J L, Labataille P
Department of Radiobiology, Cross Cancer Institute, Edmonton, Alberta, Canada.
Int J Radiat Biol. 1993 Jun;63(6):669-76. doi: 10.1080/09553009314552061.
A major product of the radiation-induced decomposition of 2'-deoxyguanosine in oxygen-saturated aqueous solution has been isolated by reverse phase high performance liquid chromatography and characterized by carbon and proton NMR spectroscopy, fast-atom bombardment mass spectrometry, and chemical analysis as (2S)-2,5'-anhydro-1-(2'-deoxy-beta-D-erythro-pentofuranosyl)-5-guanid inylidene- 2-hydroxy-4-oxoimidazolidine(d < G). This compound is stable in aqueous solution at room temperature but decomposes upon heating (45 degrees C). The lesion is also observed following type I (riboflavin, benzophenone, and acetophenone) photosensitized irradiation of 2'-deoxyguanosine at 350 nm in oxygen-saturated aqueous solution. A similar reaction mechanism, involving a neutral guanine radical intermediate, is proposed to explain the generation of d < G following both types of irradiation.
通过反相高效液相色谱法分离出了在氧饱和水溶液中2'-脱氧鸟苷辐射诱导分解的一种主要产物，并通过碳和质子核磁共振光谱、快原子轰击质谱以及化学分析将其表征为(2S)-2,5'-脱水-1-(2'-脱氧-β-D-赤藓糖戊呋喃糖基)-5-胍基亚氨基-2-羟基-4-氧代咪唑烷(d<G)。该化合物在室温下的水溶液中稳定，但加热(45摄氏度)时会分解。在氧饱和水溶液中于350nm对2'-脱氧鸟苷进行I型(核黄素、二苯甲酮和苯乙酮)光敏辐射后也观察到了这种损伤。提出了一种类似的反应机制，涉及中性鸟嘌呤自由基中间体，以解释两种辐射后d<G的产生。
