Hepatic oxygen consumption and cytochrome P450 activity in experimental faecal peritonitis.

OBJECTIVE

To study hepatic oxygen consumption and cytochrome P450 activity in pigs with septic shock induced by faecal peritonitis.

DESIGN

Controlled experimental study.

ANIMALS

12 pigs weighing 19-27 kg.

INTERVENTION

The animals were divided into a control group (n = 6) and a peritonitis group (n = 6). Peritonitis was induced by intraperitoneal instillation of a standard amount of autologous faeces. The animals were then observed for 300 minutes. Liver biopsy specimens were taken at 0 and 300 minutes.

MAIN OUTCOME MEASURES

Hepatic oxygen delivery (DO2) and consumption (VO2). Cytochrome P450 activity was studied by measuring O- and N-demethylation of codeine at 0 and 300 minutes.

RESULTS

Hepatic DO2 was reduced, whereas VO2 was increased during sepsis. There were no significant changes in the N- and O-demethylation of codeine.

CONCLUSIONS

Hepatic VO2 did increase during sepsis, possibly because of the increased metabolic demand. Cytochrome P450 activity was unaffected by the septic challenge.