β-肾上腺素能刺激诱导培养的猪颗粒细胞中3β-羟基类固醇脱氢酶（3β-HSD）mRNA积累的变化。
Alterations in 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) mRNA accumulation induced by beta-adrenergic stimulation in cultured porcine granulosa cells.
作者信息
Li X M, Juorio A V, Chedrese P J, Murphy B D
机构信息
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
出版信息
Gen Pharmacol. 1993 May;24(3):715-20. doi: 10.1016/0306-3623(93)90236-q.
PMID:8103495
Abstract
- In primary cultures of porcine granulosa cells incubation with isoproterenol (10(-7)-10(-5) M) produced a dose-dependent increase in 3 beta-HSD mRNA, 3 beta-HSD content and progesterone production which ranged from 1.5- to 5-fold. 2. These effects were completely blocked by alprenolol. Terbutaline (10(-6) M) increased 3 beta-HSD mRNA, 3 beta-HSD content and progesterone production (1.5- to 3-fold), an effect which could be prevented by alprenolol. 3. Treatment with dobutamine (10(-6) M) produced no significant change in 3 beta-HSD mRNA accumulation. 4. The results suggest that beta-adrenergic agonists have the capacity to regulate transcription and translation of 3 beta-HSD mRNA in granulosa cells and this effect is mediated by the beta 2 adrenergic receptor.
摘要
- 在猪颗粒细胞原代培养物中，与异丙肾上腺素（10⁻⁷ - 10⁻⁵ M）一起孵育会使3β-羟类固醇脱氢酶（3β-HSD）mRNA、3β-HSD含量和孕酮生成呈剂量依赖性增加，增幅在1.5至5倍之间。2. 这些效应被阿普洛尔完全阻断。特布他林（10⁻⁶ M）增加了3β-HSD mRNA、3β-HSD含量和孕酮生成（1.5至3倍），阿普洛尔可阻止这一效应。3. 用多巴酚丁胺（10⁻⁶ M）处理对3β-HSD mRNA积累没有产生显著变化。4. 结果表明，β-肾上腺素能激动剂有能力调节颗粒细胞中3β-HSD mRNA的转录和翻译，且这种效应由β₂肾上腺素能受体介导。
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