胰腺和蛇毒磷脂酶A2对人白细胞体外生成白三烯B4和C4的影响。

Effects of pancreatic and snake venom phospholipase A2 on the generation of leukotriene B4 and C4 by human leukocytes in vitro.

作者信息

Shimizu T, Hansson G C, Strandvik B

机构信息

Department of Paediatrics, University of Göteborg, Sweden.

出版信息

Pancreas. 1994 Jan;9(1):37-41. doi: 10.1097/00006676-199401000-00005.

DOI:10.1097/00006676-199401000-00005

PMID:8108369

Abstract

To investigate the effects of phospholipase A2 (PLA2) on leukotriene (LT) generation, we evaluated LTB4 and LTC4 production by human leukocytes after incubation with porcine pancreatic and snake venom PLA2. The pancreatic and venom PLA2 did not induce LTB4 and LTC4 generation by human leukocytes alone. However, both types of PLA2 promoted opsonized zymosan-induced LTB4 production in a dose-dependent manner (from 10(-10) to 10(-8) M), whereas they had no effect on opsonized zymosan-induced LTC4 generation. This promoting effect of pancreatic and venom PLA2 required Ca2+ in the incubation medium and was inhibited by mepacrine (10(-5) M). These findings suggest that increased production of PLA2 in combination with inflammatory stimuli such as endotoxin enhance LTB4 biosynthesis in acute pancreatitis.

摘要

为研究磷脂酶A2（PLA2）对白三烯（LT）生成的影响，我们评估了人白细胞在与猪胰PLA2和蛇毒PLA2孵育后白三烯B4（LTB4）和白三烯C4（LTC4）的产生情况。单独的猪胰PLA2和蛇毒PLA2不会诱导人白细胞产生LTB4和LTC4。然而，两种类型的PLA2均以剂量依赖性方式（从10^(-10)至10^(-8) M）促进调理酵母聚糖诱导的LTB4产生，而它们对调理酵母聚糖诱导的LTC4产生没有影响。猪胰PLA2和蛇毒PLA2的这种促进作用需要孵育培养基中的Ca2+，并被米帕林（10^(-5) M）抑制。这些发现表明，在内毒素等炎性刺激作用下，PLA2产量增加会增强急性胰腺炎中LTB4的生物合成。