Sodomková D, Rademacher R, Vanásek J
Vet Med (Praha). 1975 Jun;20(7):315-20.
In leukemic cows the percentage of leucocytes and lymphoid cells of the peripheral blood and marrow, with intensely stained violet glycogen granules in the plasma, which nevertheless exceptionally appear even in the leucocytes and lymphocytes of healthy cows, is significantly higher. Lymphocytes with a negative PAS reaction are found in both healthy and leukemic cows. PAS reaction can therefore be used as an auxiliary method for differentiating leukemic lymphocytes in a complex of further cytochemical reactions. In neutrophilous peripheral blood leucocytes of healthy and leucotic cattle the PAS reaction attained the values of ++ to +++. In the marrow of both groups an attenuated reaction was recorded in the myelocytes and promyelocytes (0 to +). Eosinophilous leucocytes and their developmental stages yielded negative PAS reaction. Monocytes showed a weak reaction in the periphery of healthy cattle whereas in the leucotic cattle the reaction was somewhat stronger, PAS reaction of the erythrocyte and thrombocyte bone marrow series was negative.
患白血病的奶牛外周血和骨髓中的白细胞及淋巴细胞百分比显著更高，其血浆中有强烈染色呈紫色的糖原颗粒，不过即使在健康奶牛的白细胞和淋巴细胞中也偶尔会出现这种颗粒。在健康奶牛和患白血病的奶牛中均发现了PAS反应阴性的淋巴细胞。因此，在一系列进一步的细胞化学反应中，PAS反应可作为鉴别白血病淋巴细胞的辅助方法。在健康和患白血病牛的嗜中性外周血白细胞中，PAS反应呈++至+++。在两组动物的骨髓中，髓细胞和早幼粒细胞的反应减弱（0至+）。嗜酸性白细胞及其发育阶段PAS反应呈阴性。单核细胞在健康牛外周血中反应较弱，而在患白血病的牛中反应稍强，红细胞和血小板骨髓系列的PAS反应呈阴性。
