通过悬浮培养细胞电穿孔法生产可育转基因玉米。

Production of fertile transgenic maize by electroporation of suspension culture cells.

作者信息

Laursen C M, Krzyzek R A, Flick C E, Anderson P C, Spencer T M

机构信息

Discovery Research, DEKALB Plant Genetics, Mystic, CT 06355-1958.

出版信息

Plant Mol Biol. 1994 Jan;24(1):51-61. doi: 10.1007/BF00040573.

DOI:10.1007/BF00040573

PMID:8111026

Abstract

Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.

摘要

通过对用果胶降解酶处理过的悬浮培养细胞进行电穿孔，获得了可育的转基因玉米植株。用含有bar基因的质粒对来自两种不同悬浮培养物（一种源自A188×B73，另一种源自与B73相关的自交系）的细胞进行电穿孔，结果稳定转化的愈伤组织系得以高频恢复。从13个转化的愈伤组织系再生出植株，并证明了bar基因向后代的传递。