类固醇诱导培养的嗜铬细胞中腺苷转运的抑制。
Steroid-induced inhibition of adenosine transport in cultured chromaffin cells.
作者信息
Fideu M D, Miras-Portugal M T
机构信息
Department of Biochemistry, Veterinary Faculty, Complutense University, Madrid, Spain.
出版信息
Cell Mol Neurobiol. 1993 Oct;13(5):493-502. doi: 10.1007/BF00711458.
Abstract
- Adenosine transport is subjected to regulation by hormones. Glucocorticoids, sexual steroids, and retinoic acid inhibit adenosine transport in chromaffin cells after a long-term incubation period (24 hr). No effects were observed after a short-term incubation period (10 min). 2. The kinetic parameters of transporters were studied. No significant changes were observed for the affinity constant (Km), whose value remains at 1 +/- 0.2 microM after 24-hr incubation in the presence of these compounds. The maximal velocity (Vmax) was significantly modified, with a decrease of about 20% in all cases. 3. NBTI binding was not modified in its affinity constant or maximal bound capacity (Bmax) by the presence of these compounds for a 24-hr incubation period. Thus the efficiency of transporters (quotient Vmax/Bmax) changed from 10.9 +/- 0.08 adenosine molecules transported per transporter per sec in the control cells to 9.1 +/- 0.07 in hormone-treated cultured cells. 4. The thyroid hormone (T3) significantly increased adenosine transport in a long-term incubation period in chromaffin cells (24 hr). This activatory effect is antagonized by steroid hormones and retinoic acid.
摘要
- 腺苷转运受激素调节。长期孵育（24小时）后，糖皮质激素、性类固醇和视黄酸可抑制嗜铬细胞中的腺苷转运。短期孵育（10分钟）后未观察到影响。2. 研究了转运体的动力学参数。亲和常数（Km）未观察到显著变化，在这些化合物存在下孵育24小时后，其值保持在1±0.2微摩尔。最大速度（Vmax）有显著改变，所有情况下均下降约20%。3. 对于24小时的孵育期，这些化合物的存在未改变NBTI结合的亲和常数或最大结合容量（Bmax）。因此，转运体的效率（Vmax/Bmax商）从对照细胞中每个转运体每秒转运10.9±0.08个腺苷分子变为激素处理的培养细胞中的9.1±0.07。4. 甲状腺激素（T3）在嗜铬细胞的长期孵育期（24小时）显著增加腺苷转运。这种激活作用被类固醇激素和视黄酸拮抗。
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