编码神经末梢蛋白SNAP - 25两种不同亚型的人cDNA克隆。

Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25.

作者信息

Bark I C, Wilson M C

机构信息

Scripps Research Institute, Department of Neuropharmacology, La Jolla, CA 92037.

出版信息

Gene. 1994 Feb 25;139(2):291-2. doi: 10.1016/0378-1119(94)90773-0.

DOI:10.1016/0378-1119(94)90773-0

PMID:8112622

Abstract

Two distinct cDNA sequences, corresponding to alternative isoforms of the human nerve terminal protein SNAP-25 (synaptosomal associated protein of 25 kDa), were cloned and characterized. Sequence analysis demonstrated that the two isoforms are generated by alternative splicing between two distinct but homologous exons 5, a and b each encoding 39 amino acids (aa). Although the two isoforms, SNAP-25a and SNAP-25b, differ by only 9 aa, this domain encodes the portion of the protein that is a substrate for post-translational fatty acylation, and therefore might be important for regulating subcellular localization and membrane targeting.

摘要

克隆并鉴定了两个不同的cDNA序列，它们对应于人神经末梢蛋白SNAP-25（25 kDa的突触体相关蛋白）的可变剪接异构体。序列分析表明，这两种异构体是由两个不同但同源的外显子5（a和b）之间的可变剪接产生的，每个外显子编码39个氨基酸（aa）。尽管两种异构体SNAP-25a和SNAP-25b仅相差9个氨基酸，但该结构域编码的蛋白质部分是翻译后脂肪酰化的底物，因此可能对调节亚细胞定位和膜靶向很重要。