Andley U P, Lewis R M, Reddan J R, Kochevar I E
Department of Ophthalmology and Visual Sciences, Washington, University School of Medicine, St. Louis, Missouri 63110.
Invest Ophthalmol Vis Sci. 1994 Feb;35(2):367-73.
This study was done to quantitate the biologic effects of different wavelengths of radiation in the UVA- and UVB-wavelength region on cultured rabbit lens epithelial cells.
An action spectrum for UV-induced cytotoxicity as measured by colony-forming ability was determined using six different monochromatic wavelengths from 297 to 405 nm in rabbit lens epithelial cell line N/N1003A. Cell survival was determined by clonogenic assay. Fluence rates were monitored with a calibrated radiometer.
Survival curves show that cell killing was most efficient at 297 nm. After quantum correction, the efficiency of 297-nm radiation in cell killing was 7 times greater than was 302-nm radiation. Radiation at 297 nm was more than 170, 340, 560, and 2000 times as effective in cell killing as 313-, 325-, 334-, and 365-nm radiation, respectively. The action spectrum had a shape similar to the DNA absorption spectrum in the UVB region, suggesting that DNA may be one of the critical targets for damage to the cells. At wavelengths longer than 313 nm, the shape of the action spectrum deviated from the DNA absorption spectrum.
Cytotoxicity of UV radiation in cultured lens epithelial cells varies greatly with wavelength within the UVA and UVB regions. Different mechanisms may predominate in the two wavelength regions. Cultured cells may provide a suitable system for investigating the mechanisms by which UV radiation damages lens epithelial cells and leads to cataract formation.
本研究旨在定量分析UVA和UVB波长区域内不同波长的辐射对培养的兔晶状体上皮细胞的生物学效应。
使用兔晶状体上皮细胞系N/N1003A中297至405 nm的六种不同单色波长，测定通过集落形成能力衡量的紫外线诱导细胞毒性的作用光谱。通过克隆形成试验确定细胞存活率。用校准的辐射计监测通量率。
存活曲线表明，细胞杀伤在297 nm处最有效。经过量子校正后，297 nm辐射的细胞杀伤效率比302 nm辐射高7倍。297 nm的辐射在细胞杀伤方面分别比313、325、334和365 nm的辐射有效170多倍、340多倍、560多倍和2000多倍。该作用光谱的形状与UVB区域的DNA吸收光谱相似，表明DNA可能是细胞损伤的关键靶点之一。在波长大于313 nm时，作用光谱的形状偏离了DNA吸收光谱。
培养的晶状体上皮细胞中紫外线辐射的细胞毒性在UVA和UVB区域内随波长变化很大。两个波长区域可能有不同的主导机制。培养的细胞可能为研究紫外线辐射损伤晶状体上皮细胞并导致白内障形成的机制提供一个合适的系统。
