[5-羟基二十碳四烯酸和肝氧磷脂素A3对蜗牛神经元胆碱能受体可塑性影响的比较]

[A comparison of the effects of 5-hydroxyeicosatetraenoic acid and hepoxilin A3 on the plasticity of the snail neuronal cholinoreceptors].

作者信息

Pivovarov A S, Demin P M, Miagkova G I

出版信息

Biull Eksp Biol Med. 1993 Oct;116(10):378-81.

Abstract

The effects of two acyclic derivatives of arachidonic acid which are formed under the action of 5- and 12-lipoxygenases 5(S)-hydroxy-(6,8Z,11Z,14Z)-eicosatetraenoic acid (5-HETE) and (8R/S)-hydroxy-(11S,12S)-epoxy-5Z,9E,14Z-eicosatrienoi c acid (hepoxilin A3) on extinction of inward current evoked by local acetylcholine (ACh-current) application on soma of Helix lucorum RPa3 and LPa3 neurons were studied by the double-electrode voltage clamp technique. It was shown an increase in ACh-current extinction by 5-HETE. Hepoxilin A3 did not influence cholinoreceptor plasticity. The present results confirm earlier assumptions concerning the regulation of cholinoreceptor plasticity by acyclic eicosanoids which were formed from arachidonic acid under the influence of 5-lipoxygenase and the lack of 12-lipoxygenase metabolites in this regulation.

摘要

采用双电极电压钳技术，研究了花生四烯酸的两种无环衍生物（在5-脂氧合酶和12-脂氧合酶作用下形成的5(S)-羟基-(6,8Z,11Z,14Z)-二十碳四烯酸（5-HETE）和(8R/S)-羟基-(11S,12S)-环氧-5Z,9E,14Z-二十碳三烯酸（hepoxilin A3））对在福寿螺RPa3和LPa3神经元胞体上局部施加乙酰胆碱所诱发的内向电流（ACh电流）消退的影响。结果显示，5-HETE可使ACh电流消退增加。Hepoxilin A3对胆碱能受体可塑性无影响。本研究结果证实了早期关于由花生四烯酸在5-脂氧合酶影响下形成的无环类二十烷酸对胆碱能受体可塑性的调节作用，以及在该调节过程中缺乏12-脂氧合酶代谢产物的假设。