Tomlinson A P, Van Horn H H, Wilcox C J, Harris B
Dairy Science Department, University of Florida, Gainesville 32611.
J Dairy Sci. 1994 Jan;77(1):145-56. doi: 10.3168/jds.S0022-0302(94)76937-X.
Because some previous studies indicated that addition of dietary fat may delay milk yield response and that effects carry over after withdrawal, an objective of this reversal design with four 28-d periods was to estimate residual effects. Diets were fed 2 wk before period 1 to permit inclusion of pretreatment diet in the mathematical model and changed for each of 33 cows at the start of periods 1 through 3; period 4 treatments continued those for period 3. Diets were 50% corn silage supplemented to be 12% CP with soybean meal and urea; 15% CP with soybean meal, blood and soybean meals, or feather and soybean meals; and 18% CP with soybean meal or blood and soybean meals. Protein treatments were replicated in diets containing 2.0% Ca soaps of fatty acids. No carry-over effects were significant; however, yield increases from Ca soaps were not evident until wk 4. Diet CP had a positive linear effect on milk and SCM yields, BW, and blood urea N. Milk protein percentage was higher from soybean meal diets. Addition of dietary Ca soaps of fatty acids increased milk, protein, fat, and SCM yields. Milk protein percentage was depressed when Ca soaps of fatty acids were fed with blood meal but not with soybean meal. No positive responses were observed from increasing dietary undegradable protein with blood meal or feather meal.
因为之前的一些研究表明,日粮中添加脂肪可能会延迟产奶量的反应,并且在撤掉脂肪后这种影响仍会持续,所以这个采用四个28天周期的反转设计的目的是评估残留效应。在第1周期前2周饲喂日粮,以便在数学模型中纳入预处理日粮,并在第1至3周期开始时对33头奶牛中的每头奶牛更换日粮;第4周期的处理延续第3周期的处理。日粮为50%玉米青贮,用豆粕和尿素补充至粗蛋白含量为12%;用豆粕、血粉和豆粕或羽毛粉和豆粕补充至粗蛋白含量为15%;用豆粕或血粉和豆粕补充至粗蛋白含量为18%。蛋白质处理在含有2.0%脂肪酸钙皂的日粮中重复进行。没有显著的残留效应;然而,直到第4周,脂肪酸钙皂对产奶量的增加才明显。日粮粗蛋白对牛奶和标准乳产量、体重以及血尿素氮有正线性影响。豆粕日粮的乳蛋白率更高。日粮中添加脂肪酸钙皂可提高牛奶、蛋白质、脂肪和标准乳产量。当脂肪酸钙皂与血粉一起饲喂时,乳蛋白率会降低,但与豆粕一起饲喂时则不会。用血粉或羽毛粉增加日粮中不可降解蛋白未观察到积极反应。