Schwartz A, Leng M
Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
J Mol Biol. 1994 Mar 4;236(4):969-74. doi: 10.1016/0022-2836(94)90002-7.
DNase I has been used as an enzymatic probe to visualize the conformational alteration induced in DNA by the binding of either the antitumor drug cis-platinum (cis-DDP) or the therapeutically inactive derivatives, trans-platinum (trans-DDP) and chlorodiethylene-triamineplatinum(II) (dien-Pt). We have constructed double-stranded oligonucleotides (52-mer) containing a single adduct either at the d(GG) site (cis-DDP intrastrand cross-link) or at the d(GC/GC) site (cis-DDP interstrand cross-link) or at the d(G/C) site (trans-DDP interstrand cross-link) or at the d(G) site (dien-Pt adduct). The platinated oligonucleotides are differently recognized by DNase I. As judged by DNase I, the distortions induced in the DNA double helix by the cis-DDP and trans-DDP interstrand cross-links spread over more base-pairs than that induced by the cis-DDP intrastrand cross-link.
脱氧核糖核酸酶I（DNase I）已被用作一种酶促探针，以观察由抗肿瘤药物顺铂（cis-DDP）或治疗无活性衍生物反铂（trans-DDP）和二氯二乙三胺铂（II）（dien-Pt）的结合所诱导的DNA构象改变。我们构建了双链寡核苷酸（52聚体），其在d(GG)位点（顺铂链内交联）、d(GC/GC)位点（顺铂链间交联）、d(G/C)位点（反铂链间交联）或d(G)位点（dien-Pt加合物）含有单个加合物。铂化寡核苷酸被DNase I以不同方式识别。根据DNase I判断，顺铂和反铂链间交联在DNA双螺旋中诱导的扭曲比顺铂链内交联诱导的扭曲扩展到更多碱基对。
