福岛医科大学医院分离出的耐甲氧西林金黄色葡萄球菌的特性及mec A基因

The properties and mec A gene of the methicillin-resistant Staphylococcus aureus isolated in Fukushima Medical College Hospital.

作者信息

Tanabe F, Sato T, Nozawa A, Nihonmatsu H, Okamura H, Imafuku Y, Ogata M, Yoshida H

机构信息

Department of Clinical Laboratory Medicine, Fukushima Medical College, Japan.

出版信息

Fukushima J Med Sci. 1993 Jun;39(1):35-42.

PMID:8125428

Abstract

The 106 methicillin-resistant strains of Staphylococcus aureus (MRSA) isolated in Fukushima Medical College Hospital were examined for their properties and mecA gene. The strains produced four types of coagulase, of which type II was the most common, produced by 84 (79.2%). Beta-lactamase was produced by 58 (50%). Enterotoxins were produced by 45 (42.5%), most of which (39/106, 36.8%) were of type A. Thirty-four strains (32.1%) produced both enterotoxins and toxic shock syndrome toxin-1. All strains were susceptible to vancomycin and arbekacin, although they were mostly resistant to many other antibiotics. Using the polymerase chain reaction (PCR) technique, the mecA gene was detected in 57 (91.9%) of the 62 strains used. In addition, one of the 42 methicillin-susceptible strains isolated had the mecA gene. These results indicate that detection of the mecA gene by the PCR technique is a rapid and accurate way to identify methicillin resistance.

摘要

对福岛医学院附属医院分离出的106株耐甲氧西林金黄色葡萄球菌（MRSA）进行了特性及mecA基因检测。这些菌株产生四种凝固酶，其中II型最为常见，84株（79.2%）产生该型凝固酶。58株（50%）产生β-内酰胺酶。45株（42.5%）产生肠毒素，其中大部分（39/106，36.8%）为A型。34株（32.1%）同时产生肠毒素和中毒性休克综合征毒素-1。所有菌株对万古霉素和阿贝卡星敏感，尽管它们大多对许多其他抗生素耐药。使用聚合酶链反应（PCR）技术，在所检测的62株菌株中有57株（91.9%）检测到mecA基因。此外，在分离出的42株甲氧西林敏感菌株中有1株含有mecA基因。这些结果表明，通过PCR技术检测mecA基因是鉴定甲氧西林耐药性的一种快速且准确的方法。