Barleon B, Hauser S, Schöllmann C, Weindel K, Marmé D, Yayon A, Weich H A
Institute of Molecular Cell Biology, University of Freiburg, Germany.
J Cell Biochem. 1994 Jan;54(1):56-66. doi: 10.1002/jcb.240540107.
Vascular endothelial growth factor (VEGF) is a newly identified growth and permeability factor with a unique specificity for endothelial cells. Recently the flt-encoded tyrosine kinase was characterized as a receptor for VEGF. A novel tyrosine kinase receptor encoded by the KDR gene was also found to bind VEGF with high affinity when expressed in CMT-3 cells. Screening for flt and KDR expression in a variety of species and tissue-derived endothelial cells demonstrates that flt is predominantly expressed in human placenta and human vascular endothelial cells. Placenta growth factor (PIGF), a growth factor significantly related to VEGF, is coexpressed with flt in placenta and human vascular endothelial cells. KDR is more widely distributed and expressed in all vessel-derived endothelial cells. These data demonstrate that cultured human endothelial cells isolated from different tissues express both VEGF receptors in relative high levels and, additionally, that all investigated nonhuman endothelial cells in culture are also positive for KDR gene expression.
血管内皮生长因子（VEGF）是一种新发现的生长和通透性因子，对内皮细胞具有独特的特异性。最近，flt编码的酪氨酸激酶被鉴定为VEGF的受体。当在CMT-3细胞中表达时，由KDR基因编码的一种新型酪氨酸激酶受体也被发现能与VEGF高亲和力结合。对多种物种和组织来源的内皮细胞中flt和KDR表达的筛选表明，flt主要在人胎盘和人血管内皮细胞中表达。胎盘生长因子（PIGF）是一种与VEGF显著相关的生长因子，在胎盘和人血管内皮细胞中与flt共表达。KDR分布更广泛，在所有血管来源的内皮细胞中均有表达。这些数据表明，从不同组织分离的培养人内皮细胞相对高水平地表达两种VEGF受体，此外，所有研究的培养非人类内皮细胞的KDR基因表达也呈阳性。
