Ezaki T
Department of Microbiology, Gifu University School of Medicine.
Nihon Rinsho. 1994 Feb;52(2):355-8.
Conventional biochemical characterization system of pathogenic bacteria spent too much time to identify pathogens. However, the introduction of new genetic technique has pushed these classical methods aside and rapid determination of pathogenic bacteria in infectious disease become possible. Quantitative DNA/DNA hybridization, Specific DNA probe, and PCR techniques are developed for the identification and detection of almost all medically important pathogens. This development was mainly brought by the accumulation of 16S rRNA sequences of bacteria. From conserved area of 16S rRNA and 18S rRNA sequence, universal PCR primers are designed and universal bacterial and fungal detection system are applied for the rapid diagnosis of causative agent of sepsis and meningitis.
传统的病原菌生化鉴定系统鉴定病原菌耗时过长。然而，新基因技术的引入将这些经典方法抛在了一边，使传染病中病原菌的快速鉴定成为可能。定量DNA/DNA杂交、特异性DNA探针和PCR技术已被开发用于几乎所有医学上重要病原菌的鉴定和检测。这一进展主要得益于细菌16S rRNA序列的积累。从16S rRNA和18S rRNA序列的保守区域设计通用PCR引物，并应用通用细菌和真菌检测系统快速诊断败血症和脑膜炎的病原体。
