Goldburt V, Sabban B A, Kleinhaus A L
Department of Cell Biology and Anatomy, New York Medical College, Valhalla 10595.
Behav Neural Biol. 1994 Jan;61(1):47-53. doi: 10.1016/s0163-1047(05)80043-3.
The effect of reserpine on the serotonin content of the central nervous system and the feeding behavior of the carnivorous leech Haemopis marmorata was investigated. Leeches were hand-fed to satiation by presentation of pieces of chicken liver held in forceps for three meals at 4-day intervals prior to and 1 week following three consecutive injections of 100 micrograms reserpine in the crop. A group matched by weight and preinjection food intake was injected with the vehicle in a blind experimental design. Histochemical and chemical determinations showed that reserpine effectively depleted the serotonin content of the animal's central nervous system. Furthermore, the food intake of reserpine-treated animals was significantly less than that of the sham-injected group. However, the depleted animals continued to ingest approximately 40% of the amount consumed during the preinjection period. These results show that reserpine successfully depleted the serotonin in the carnivorous leech H. marmorata and decreased the food intake of this leech species. They suggest that feeding behavior in Haemopis is partially but not exclusively dependent on serotonin and that, in contrast to the sanguivorous leeches, additional factors may be necessary for its regulation.
研究了利血平对肉食性水蛭（Haemopis marmorata）中枢神经系统中血清素含量及摄食行为的影响。在连续三次向嗉囊中注射100微克利血平之前4天以及之后1周，每隔4天用镊子夹着鸡肝块人工喂食水蛭至饱足，共喂食三餐。在一项盲法实验设计中，将一组体重和注射前食物摄入量匹配的水蛭注射赋形剂。组织化学和化学测定表明，利血平有效地耗尽了动物中枢神经系统中的血清素含量。此外，经利血平处理的动物的食物摄入量明显低于假注射组。然而，血清素耗尽的动物继续摄入约为注射前时期消耗量40%的食物量。这些结果表明，利血平成功地耗尽了肉食性水蛭H. marmorata中的血清素，并降低了这种水蛭的食物摄入量。它们表明，Haemopis的摄食行为部分但并非完全依赖于血清素，并且与吸血水蛭相比，其调节可能还需要其他因素。
