黑腹果蝇的卵壳。八、卵子发生过程中蜡层的形态发生。

The eggshell of Drosophila melanogaster. VIII. Morphogenesis of the wax layer during oogenesis.

作者信息

Papassideri I S, Margaritis L H, Gulik-Krzywicki T

机构信息

Department of Biochemistry, Cell and Molecular Biology and Genetics, University of Athens, Greece.

出版信息

Tissue Cell. 1993 Dec;25(6):929-36. doi: 10.1016/0040-8166(93)90041-i.

DOI:10.1016/0040-8166(93)90041-i

PMID:8140583

Abstract

Utilizing freeze-fracturing and conventional electron microscopy methods, we have studied the details of morphogenesis and construction of the wax layer envelope from Oregon R and mutants of Drosophila melanogaster eggs during oogenesis. The wax layer is synthesized and secreted by the follicular cells in the form of lipid vesicles during stage 10b. During secretion (stages 10b, 11 and 12) the lipid vesicles are accumulated on the vitelline membrane surface and become flat. At the late stages of choriogenesis (stages 13, 14) the lipid vesicles are compressed tightly between the vitelline membrane and the other already constructed eggshell layers, so the wax layer becomes very thin and is hardly seen in cross-fractured views.

摘要

利用冷冻断裂和传统电子显微镜方法，我们研究了黑腹果蝇卵母细胞发生过程中，俄勒冈R品系及突变体卵蜡层包膜的形态发生和结构细节。蜡层在10b期由滤泡细胞以脂质小泡的形式合成并分泌。在分泌过程中（10b期、11期和12期），脂质小泡聚集在卵黄膜表面并变平。在成壳后期（13期、14期），脂质小泡被紧密压缩在卵黄膜和其他已构建的卵壳层之间，因此蜡层变得非常薄，在交叉断裂视图中几乎看不到。