Romert L, Jansson T, Jenssen D
Genotox AB, Stockholm, Sweden.
Toxicol Lett. 1994 Mar;71(1):39-46. doi: 10.1016/0378-4274(94)90196-1.
In this study, 50 chemicals selected on the basis of existence of particularly reliable human toxicity data were screened in a cytotoxicity test involving inhibition of the growth of Ascites Sarcoma BP8 cells. These test results are part of an international validation program, the Multicenter Evaluation of In Vitro Cytotoxicity (MEIC), the aim of which is to recommend batteries of in vitro tests to be used for prediction of human toxicity. The cytotoxicities (expressed as the concentrations causing 50% inhibition of cell growth) were compared to acute toxicity data in humans (LDL0) and rodents (LD50), showing the best correlation to rodent data. The results are discussed in relationship to what is usually referred to as basal cytotoxic mechanisms as a cause of in vivo toxicity. It could be concluded that the predicted results on the basis of mechanistic reasoning were not always obtained.
在本研究中，基于特别可靠的人体毒性数据的存在而选择的50种化学物质，在一项涉及抑制腹水肉瘤BP8细胞生长的细胞毒性试验中进行了筛选。这些测试结果是国际验证计划“体外细胞毒性多中心评估（MEIC）”的一部分，其目的是推荐用于预测人体毒性的体外测试组合。将细胞毒性（表示为引起50%细胞生长抑制的浓度）与人体（LDL0）和啮齿动物（LD50）的急性毒性数据进行比较，结果显示与啮齿动物数据的相关性最佳。结合通常被称为基础细胞毒性机制作为体内毒性原因的情况对结果进行了讨论。可以得出结论，基于机制推理的预测结果并非总能得到。
