Meissner H P, Atwater I J
Horm Metab Res. 1976 Jan;8(1):11-6. doi: 10.1055/s-0028-1093685.
The effect of a "square wave" stimulation with glucose or glibenclamide on the electrical activity of beta-cells has been studied with microelectrode techniques in isolated perifused mouse islets. While glucose evoked a burst activity with periodic oscillations of the membrane potential between two levels, glibenclamide produced a constant depolarization with a continuous spike activity. In both cases the time course of activity was biphasic and resembled closely the corresponding patterns of insulin release. The effect of glibenclamide on the membrane was irreversible. The results provide further evidence for a direct correlation between insulin release and electrical activity. Furthermore, it is suggested that the membrane potential is, at least in part, involved in the mechanism of biphasic insulin release.
利用微电极技术，在分离的经灌注的小鼠胰岛中研究了葡萄糖或格列本脲的“方波”刺激对β细胞电活动的影响。葡萄糖诱发了爆发性活动，膜电位在两个水平之间周期性振荡，而格列本脲则产生持续去极化并伴有连续的锋电位活动。在这两种情况下，活动的时间进程都是双相的，并且与相应的胰岛素释放模式非常相似。格列本脲对膜的作用是不可逆的。这些结果为胰岛素释放与电活动之间的直接相关性提供了进一步的证据。此外，提示膜电位至少部分参与了双相胰岛素释放的机制。
