Horvath C M, Wolven A, Machadeo D, Huber J, Boter L, Benedetti M, Hempstead B, Chao M V
Department of Cell Biology and Anatomy, Cornell University Medical College, New York 10021.
J Cell Sci Suppl. 1993;17:223-8. doi: 10.1242/jcs.1993.supplement_17.31.
Nerve growth factor (NGF) represents a family of structurally related trophic factors, including brain-derived neurotrophin factor (BDNF), neurotrophin-3 (NT-3), NT-4, and NT-5. These neurotrophin factors interact with two classes of receptors, the trk receptor tyrosine kinase family, and the low affinity p75 neurotrophin receptor. To study potential ligand-receptor interactions, recombinant trk fusion proteins have been constructed, and pan-trk polyclonal antisera directed against the cytoplasmic tyrosine kinase domain have been generated. The recombinant proteins were assessed for in vitro kinase activity and for the ability of K-252a to inhibit phosphorylation. Antibodies made against the fusion protein recognize all trk family members, and are effective in immunoprecipitation of affinity-crosslinked receptors. Comparative crosslinking indicates that NGF can recognize all trk receptor members, illustrating the large number of potential ligand-receptor interactions between neurotrophins and their receptors.
神经生长因子（NGF）代表一类结构相关的营养因子家族，包括脑源性神经营养因子（BDNF）、神经营养素-3（NT-3）、NT-4和NT-5。这些神经营养因子与两类受体相互作用，即trk受体酪氨酸激酶家族和低亲和力p75神经营养因子受体。为了研究潜在的配体-受体相互作用，已构建了重组trk融合蛋白，并产生了针对细胞质酪氨酸激酶结构域的泛trk多克隆抗血清。对重组蛋白进行了体外激酶活性以及K-252a抑制磷酸化能力的评估。针对融合蛋白制备的抗体可识别所有trk家族成员，并在亲和交联受体的免疫沉淀中有效。比较交联表明，NGF可识别所有trk受体成员，这说明了神经营养因子与其受体之间存在大量潜在的配体-受体相互作用。
